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RNA Interference
D. Engelke

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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.RNA interference (RNAi) is a recently-developed research tool, which allows scientists to knock out or knock down the levels of a gene product, shut off targeted genes in an organism, and then monitor the results within that organism. With contributions from leading academics and biotechnology researchers, this book offers protocols and advice for the use of RNA interference in a number of different eukaryotes. Tools for the Arabidopsis, Caenrhabditis, and Drosophila model organism systems are offered, as is an overview of siRNA design and RNA transfection processes in mammalian cells. A discussion of chemical and enzymatic synthesis and preparation of siRNA in vitro is also included.